The Hidden Effects: How Herbicides Influence Soybean Yield and Physiology

Exploring the complex relationship between chemical weed control and crop performance

Soybean Research Agricultural Science Crop Physiology

More Than Just Weed Killers: The Complex Relationship Between Herbicides and Soybeans

Soybean, often called the "miracle crop," provides a crucial source of protein and oil worldwide, forming the foundation of countless food products and animal feeds. As one of the most extensively cultivated crops globally, its productivity impacts everything from food security to agricultural economies.

Global Importance

Soybean is a fundamental crop for global food security and agricultural economies, making its productivity crucial.

Complex Interactions

Herbicides affect soybeans in multidimensional ways beyond simple weed elimination, influencing physiological processes.

Key Insight

The relationship between herbicides and crops extends beyond weed control to directly influence photosynthesis, protein production, and reproductive development in soybeans.

A Tale of Two Strategies: Pre-Emergence vs. Post-Emergence Herbicides

Pre-Emergence Herbicides

Applied before weeds sprout, creating a chemical barrier at the soil surface that intercepts weed seedlings during germination.

  • Early weed control advantage
  • Critical competitive edge for soybeans
  • Risk of early-season injury

Research shows pendimethalin tank-mixed with imazethapyr proved superior against weeds 1

Post-Emergence Herbicides

Target already-established weeds, allowing assessment of weed pressure before treatment with visible problem-solution approach.

  • Targeted application timing
  • Addresses specific weed flushes
  • Demands careful timing to avoid crop damage

Harvest-aid herbicides applied too early can severely reduce soybean yield 2

Herbicide Application Timing and Effects

The Ripple Effect: How Herbicides Impact Soybean Reproduction

Groundbreaking Study on Auxin Mimics

Researchers investigated how sublethal rates of auxin mimic herbicides influence soybean reproduction and nutritional value for pollinators 3 . Applications at fractions of recommended field rates (as low as 1/100x) during early vegetative stages revealed significant impacts on reproductive development.

Surprising Findings and Ecological Implications

Dicamba and florpyrauxifen-benzyl at just 1/100x of the labeled rate reduced total reproductive organs by 31% and 27% respectively, and pollen grains per anther by 25% and 18% 3 . These changes translated directly into yield reductions of 24% and 11%.

Impact of Sublethal Herbicide Rates on Soybean Reproduction
Herbicide Application Rate Flower/Pod Reduction Pollen Reduction Yield Impact
Dicamba 1/100x labeled rate 31% 25% 24% reduction
Florpyrauxifen-benzyl 1/100x labeled rate 27% 18% 11% reduction
2,4-D 1/100x labeled rate No significant effect No significant effect No significant effect
Quinclorac 1/100x labeled rate No significant effect No significant effect No significant effect
Yield Impact of Sublethal Herbicide Applications

Beyond Weed Control: Physiological Changes in Soybeans

Photosynthesis

Herbicides influence chlorophyll content and photosynthetic efficiency, affecting overall plant growth and development 1 .

Biomass Allocation

Dry matter partitioning between stems, leaves, and reproductive organs follows predictable patterns throughout growth stages 3 .

Protein & Oil Composition

Herbicide applications can influence protein levels in seeds while generally not affecting oil content .

Environmental Interactions

Cover crops and mulching practices influenced soybean protein and oil content more than reduced herbicide rates did, highlighting the importance of considering the entire production system 5 .

The Farmer's Dilemma: Balancing Weed Control and Crop Injury

The PPO Herbicide Case Study

PPO-inhibiting herbicides like sulfentrazon, flumioxazin, and saflufenacil provide excellent weed control but can cause significant early-season soybean injury and stand loss 4 . This creates a challenging risk-reward calculation for farmers.

Weather conditions during and after emergence play the most significant role in determining the extent of crop injury. Fields with poor drainage or ponding risk show particularly high vulnerability to herbicide injury 4 .

Important Finding: Despite visible damage, most treatments recovered by 28 days after emergence, and herbicide treatments did not negatively affect soybean yield 4 .
Risk Factors
  • Cool, wet emergence conditions
  • Poorly drained fields
  • Ponding risk areas
  • Specific herbicide chemistry
Herbicide Efficacy and Safety Profile
Herbicide/Treatment Weed Control Efficacy Crop Safety Key Considerations
Pendimethalin + Imazethapyr Superior Good More effective than pendimethalin alone 1
Ethalfluralin High Good Recommended at 2 L.ha⁻¹ for effective control
PPO-inhibitors High Causes early injury Recovery by 28 days; no yield impact 4
Dicamba (sublethal) N/A Poor Reproductive development affected 3

The Scientist's Toolkit: Researching Herbicide Effects

Germination & Vigor Testing

Scientists evaluate how herbicides affect soybean seed viability and seedling development by tracking germination percentages and measuring hypocotyl and primary root lengths 2 .

Glyphosate applications at certain growth stages can reduce normal seedling percentages

Chlorophyll & Protein Analysis

Using specialized protocols like the Lowry method for protein measurement and Hilcox-Israelstam techniques for chlorophyll extraction 1 .

These measurements reveal how herbicides influence fundamental plant processes

Growth Stage Application

Researchers apply herbicides at standardized growth stages (R3-R8) to determine crop sensitivity at different developmental phases 2 .

Soybean sensitivity decreases as plants mature

Biomass Tracking

By regularly harvesting plant samples and separating them into stems, leaves, and reproductive structures 3 .

Documents how herbicides influence dry matter accumulation

Research Methods for Assessing Herbicide Impacts
Research Method What It Measures Key Findings
Reproductive organ counting Flower and pod production Dicamba reduced reproductive organs by 31% at 1/100x rate 3
Pollen grain counting Pollen grains per anther Florpyrauxifen-benzyl reduced pollen by 18% 3
Dry matter partitioning Biomass allocation to plant parts No herbicide effect on partitioning patterns 3
Seed germination tests Seed viability and normal development Glyphosate reduced normal seedlings at R5-R7 stages 2

Conclusion: Navigating the Complex World of Herbicides

The relationship between herbicides and soybeans extends far beyond simple weed control. These chemical tools interact with crop physiology in nuanced ways—influencing everything from photosynthetic efficiency and protein content to flower formation and pollen production.

The emerging picture reveals a sophisticated interplay where herbicide chemistry, application timing, environmental conditions, and genetic factors collectively determine outcomes. While herbicides remain essential for profitable soybean production, their optimal use requires understanding these complex interactions.

References