Research Articles
Optimizing ddPCR for Ultra-Sensitive Low VAF Detection: A Guide for Biomedical Researchers
Droplet Digital PCR (ddPCR) has emerged as a powerful tool for the absolute quantification of nucleic acids, offering exceptional sensitivity for detecting low variant allele frequencies (VAF) crucial in oncology,...
Absolute Quantification of Mutant Allele Frequency: Techniques, Applications, and Clinical Impact in Precision Oncology
This article provides a comprehensive overview of the principles, methodologies, and clinical applications of absolute mutant allele frequency quantification for researchers and drug development professionals.
Droplet Digital PCR in Pancreatic Ductal Adenocarcinoma: A Comprehensive Guide for Research and Translational Application
This article provides a comprehensive overview of the application of droplet digital PCR (ddPCR) in Pancreatic Ductal Adenocarcinoma (PDAC), tailored for researchers, scientists, and drug development professionals.
Tumor-Informed vs. Tumor-Uninformed ddPCR Assays: A Comparative Guide for Precision Oncology Research
This article provides a comprehensive analysis of tumor-informed and tumor-uninformed (agnostic) approaches for circulating tumor DNA (ctDNA) detection using droplet digital PCR (ddPCR).
ddPCR for TKI Response Monitoring: A Precision Tool for Guiding Treatment-Free Remission in CML
This article explores the transformative role of droplet digital PCR (ddPCR) in monitoring tyrosine kinase inhibitor (TKI) response, with a specific focus on chronic myeloid leukemia (CML).
Longitudinal ctDNA Monitoring in NSCLC: A Comprehensive Guide for Researchers and Drug Developers
Longitudinal circulating tumor DNA (ctDNA) monitoring is transforming the management of non-small cell lung cancer (NSCLC), offering a non-invasive window into tumor dynamics.
Liquid Biopsy Revolution: ctDNA Detection for Early-Stastage Breast Cancer Management and Drug Development
Circulating tumor DNA (ctDNA) analysis represents a transformative, non-invasive tool in the management of early-stage breast cancer, offering real-time insights into tumor dynamics.
Multiplex Droplet Digital PCR for ctDNA Analysis: A Comprehensive Guide for Advanced Cancer Research and Biomarker Development
This article provides a comprehensive exploration of multiplex droplet digital PCR (ddPCR) for circulating tumor DNA (ctDNA) analysis, a transformative technology in precision oncology.
Advanced ddPCR Assay Design for KRAS Mutation Detection: A Comprehensive Guide from Principles to Clinical Applications
This article provides a comprehensive guide for researchers and drug development professionals on designing and implementing droplet digital PCR (ddPCR) assays for detecting KRAS mutations, a critical oncogenic driver in...
Optimizing Plasma Processing for cfDNA Extraction: A Complete Guide for Robust Liquid Biopsy Workflows
This article provides a comprehensive guide for researchers and drug development professionals on establishing reliable plasma processing workflows for cell-free DNA (cfDNA) extraction.